According to a spokesperson for the prefecture, an investigation has commenced to determine the cause of this alarming incident, with law enforcement ruling out any intent behind the collision. Eyewitness Johan recounted the frightening moment, stating, “We heard a big impact, a big bang," as one of the trams moved backward with speed. Additional witnesses reported the violent nature of the collision, including the tram doors being catapulted off upon impact.

In response to the situation, the Bas-Rhin region's Fire and Rescue Service took to social media, advising the public to steer clear of the area to facilitate emergency response efforts. Service director Rene Cellier noted that while the majority of the injuries were considered non-life threatening, they included trauma-related injuries such as scalp lacerations, clavicle fractures, and knee sprains. Furthermore, around 100 individuals without immediate injuries were also being treated for concerns related to the incident.

A significant emergency response was mobilized, comprising roughly 50 vehicles and 130 firefighters. Cellier emphasized that the circumstances could have led to far greater casualties. Emmanuel Auneau, director of the CTS, which oversees public transportation in Strasbourg, confirmed that while both tram drivers were not physically harmed, they were deeply traumatized by the event.